Regulatory

Part:BBa_K1686098:Design

Designed by: Nicolas Boisset   Group: iGEM15_Bordeaux   (2015-09-16)


His-Gal promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 21
    Illegal suffix found in sequence at 2048
    Illegal PstI site found at 1163
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 21
    Illegal SpeI site found at 2049
    Illegal PstI site found at 1163
    Illegal PstI site found at 2063
    Illegal NotI site found at 27
    Illegal NotI site found at 2056
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 21
    Illegal BglII site found at 1373
    Illegal BamHI site found at 2006
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 21
    Illegal suffix found in sequence at 2049
    Illegal PstI site found at 1163
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 21
    Illegal XbaI site found at 36
    Illegal SpeI site found at 2049
    Illegal PstI site found at 1163
    Illegal PstI site found at 2063
    Illegal AgeI site found at 1536
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 725


Design Notes

We used a technique called Gibson assembly to clone the biobrick in pSB1C3.

Source

This biobrick has been taken from the plasmid pFA6a-His3MX6-PGAL1-3HA.

References

Longtine_et_al-1998-Yeast